Biomembranes Part I: Visual Pigments and Purple Membranes II - download pdf or read online

By Lester Packer (Eds.)

ISBN-10: 0076687988

ISBN-13: 9780076687985

ISBN-10: 0121819884

ISBN-13: 9780121819880

The severely acclaimed laboratory general, Methods in Enzymology, is among the so much hugely revered guides within the box of biochemistry. on account that 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless proper this day - actually a vital ebook for researchers in all fields of existence sciences

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Extra info for Biomembranes Part I: Visual Pigments and Purple Membranes II

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Khim. l, 113 (1975). 3 L. A. Drachev, V. N. Frolov, A. D. Kaulen, E. A. Liberman, S. A. Ostroumov, V. G. Plakunova, A. Yu. Semenov, and V. P. Skulachev, J. Biol. Chem. 251, 7059 (1976). 4 V. P. Skulachev, FEBS Lett. 64, 23 (1976). 5 L. A. Drachev, A. D. Kaulen, A. Yu. Semenov, I. I. Severina, and V. P. Skulachev, Anal. Biochem. 96, 250 (1979). 6 V. P. Skulachev, this series, Vol. 55, pp. 586 and 751. METHODS IN ENZYMOLOGY,VOL, 88 Copyright© 1982by AcademicPress, Inc. All rightsof reproductionin any formreserved.

Then H ÷ ion (or any other cation) diffuses through the planar membrane to the bulk solution on the opposite side of the planar membrane. Thus a transmembrane continuous current and a potential difference arise. They can be measured L. A. Drachev, A. A. Jasaitis, A. , Kaulen, A. A. Kondrashin, E. A. Liberman, I. B. Nemeeek, S. A. Ostroumov, A. Yu. Semenov, and V. P. Skulachev, Nature (London) 249, 321 (1974). 2 E. L. Barsky, L. A. Drachev, A. D. Kaulen, A. A. Kondrashin, E. A. Liberman, S. A. Ostroumov, V.

2. Reconstitution Methods Employing Deoxycholate Purified Bacteriorhodopsin Bacteriorhodopsin (5-10 mg in 1 ml) is solubilized in 10% sodium deoxycholate for 24 hr at room temperature. 38 rotor of Kontron-TGA-65 ultracentrifuge: Kontron, Zfirich). The purple band is collected and used for further reconstitutions. 25 E. Racker, B. Violand, S. O. Neal, M. Alfonzo, and J. Telford,Arch. Biochem. Biophys. 198, 470 (1979). 26 E. Racker and W. Stoeckenius, J. Biol. Chem. 249, 662 (1974). 24 BACTERIORHODOPSIN [4] The amount of DOC still associated with the protein is determined by using [ZH]deoxycholate and corresponds to 7 mol of detergent per mole of bacteriorhodopsin.

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Biomembranes Part I: Visual Pigments and Purple Membranes II by Lester Packer (Eds.)

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