By Bernhard Sonnleitner
With contributions via quite a few specialists
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Additional resources for Bioanalysis and Biosensors for Bioprocess Monitoring
The determinations are mainly based on the detection of simple co-substrates and products such as O2 , H 2O2 , NH3 , or CO2 . Amperometric transducers measure the current (flux of electrons) caused by oxidation or reduction of the species of interest when a voltage is applied between the working and the reference electrode. g. g. . Other determinations focus on the Instrumentation of Biotechnological Processes 33 detection of H2 O2 or NADH . Sensors are currently being made smaller and smaller [297, 298].
Useful fields are gravity, temperature, cross flow, electrical charge, and others [128–131]. The range of the (molecular) size of the analytes usually exceeds that which can be determined by classical laboratory analytical methods such as size exclusion chromatography, etc. . Reports on investigated substances are widespread and cover applications such as the separation and characterization of proteins  and enzymes [240, 241], of viruses , the separation of human and animal cells [50, 51], the isolation of plasmid DNA , and the molecular weight and particle size distribution of polymers [216, 217].
In addition, the technical designs of injection valves differ and the injector to a gas chromatograph is heated to 200 or 250 °C which means it needs, therefore, a special construction Instrumentation of Biotechnological Processes 29 retention in the column is determined by the interaction between the individual constituents and the stationary phase. Miniaturized versions using micromachined instruments  or array detectors  have recently been reported. In the following, a non-exhaustive list of some examples is given to illustrate the versatility of these methods.
Bioanalysis and Biosensors for Bioprocess Monitoring by Bernhard Sonnleitner