Bioanalysis and Biosensors for Bioprocess Monitoring - download pdf or read online

By Bernhard Sonnleitner

ISBN-10: 3540660526

ISBN-13: 9783540660521

With contributions via quite a few specialists

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The determinations are mainly based on the detection of simple co-substrates and products such as O2 , H 2O2 , NH3 , or CO2 [142]. Amperometric transducers measure the current (flux of electrons) caused by oxidation or reduction of the species of interest when a voltage is applied between the working and the reference electrode. g. g. [169]. Other determinations focus on the Instrumentation of Biotechnological Processes 33 detection of H2 O2 or NADH [9]. Sensors are currently being made smaller and smaller [297, 298].

Useful fields are gravity, temperature, cross flow, electrical charge, and others [128–131]. The range of the (molecular) size of the analytes usually exceeds that which can be determined by classical laboratory analytical methods such as size exclusion chromatography, etc. [351]. Reports on investigated substances are widespread and cover applications such as the separation and characterization of proteins [450] and enzymes [240, 241], of viruses [132], the separation of human and animal cells [50, 51], the isolation of plasmid DNA [367], and the molecular weight and particle size distribution of polymers [216, 217].

In addition, the technical designs of injection valves differ and the injector to a gas chromatograph is heated to 200 or 250 °C which means it needs, therefore, a special construction Instrumentation of Biotechnological Processes 29 retention in the column is determined by the interaction between the individual constituents and the stationary phase. Miniaturized versions using micromachined instruments [90] or array detectors [425] have recently been reported. In the following, a non-exhaustive list of some examples is given to illustrate the versatility of these methods.

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Bioanalysis and Biosensors for Bioprocess Monitoring by Bernhard Sonnleitner


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