Read e-book online Basic gas chromatography PDF

By Harold M. McNair, James M. Miller

ISBN-10: 0470439548

ISBN-13: 9780470439548

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25-mm fused silica tubing. Such columns are properly called “wallcoated open tubular” or simply WCOT columns. Since the tube is open, its resistance to flow is very low; therefore, long lengths, up to 100 m, are possible. These long lengths permit very efficient separations of complex sample mixtures. Fused silica capillary columns are the most inert. Open tubular (OT) columns are covered in detail in Chapter 6. 2 compares these two main types of column and lists their advantages, disadvantages, and some typical characteristics.

Obviously, purchasing the carrier gas of adequate purity is not economically feasible for most laboratories. The more common practice is to purchase the High-purity grade and purify it. Water and trace hydrocarbons can be easily removed by installing a 5-Å molecular sieve filter between the gas cylinder and the instrument. Drying tubes are commercially available, or they can be readily made by filling a 6-ft by 1/4-in. column with GC grade 5-Å molecular sieve. In either case, after two gas cylinders have been used, the sieve should be regenerated by heating to 300 °C for 3 hours with a slow flow of dry nitrogen.

Chrompack International B. , Middleburg, the Netherlands, personal communication, 1996. 12. Berger, T. , Chromatographia 42, 63 (1996). CHAPTER 2 INSTRUMENT OVERVIEW Instrumentation in gas chromatography has continually evolved since the introduction of the first commercial systems in 1954. The basic components of a typical, modern gas chromatographic system are discussed individually in this chapter. 1 shows schematically a gas chromatographic system. The components that will be discussed include: (1) carrier gas, (2) flow control, (3) sample inlet and sampling devices, (4) columns, (5) controlled temperature zones (ovens), (6) detectors, and (7) data systems.

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Basic gas chromatography by Harold M. McNair, James M. Miller


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