Read e-book online Animal Cell Biotechnology: Methods and Protocols (Methods in PDF

By Ralf Pörtner

ISBN-10: 1588296601

ISBN-13: 9781588296603

ISBN-10: 1597453994

ISBN-13: 9781597453998

Animal cellphone Biotechnology: tools and Protocols, moment variation constitutes a entire handbook of state of the art and new concepts for constructing mammalian mobile strains for creation of biopharmaceuticals, and for optimizing severe parameters for telephone tradition contemplating the total cascade from lab to ultimate construction. the amount is split into 5 elements that mirror the procedures required for various levels of creation. partly I, easy suggestions for institution of construction telephone traces are addressed, specifically transduction concepts, cells for gene remedy and antibody construction. half II addresses simple cultivation ideas, comparable to microcarrier tradition and encapsulation.

Part III covers telephone characterization and research, together with stream cytometric purposes, NMR-based ideas, and biochemical and cytometric strategies. half IV information cultivation strategies, reminiscent of disposable bioreactors, hole fiber phone tradition, mounted mattress reactors, and configuration of bioreactors. half V covers downstream options similar to membrane filtration strategies, whereas half VI describes distinct functions, together with retroviral vectors.

Animal mobile Biotechnology: tools and Protocols, moment variation presents a compendium of recommendations for scientists in commercial and study laboratories that use mammalian cells for biotechnology purposes.

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Additional resources for Animal Cell Biotechnology: Methods and Protocols (Methods in Biotechnology)

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Et al. (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263–267. 20 Fluri and Fussenegger 16. Bukovsky, A. , Song, J. , and Naldini, L. (1999) Interaction of human immunodeficiency virus-derived vectors with wild-type virus in transduced cells. J. Virol. 73, 7087–7089. 17. Danthinne, X. and Imperiale, M. J. (2000) Production of first generation adenovirus vectors: a review. Gene Ther. 7, 1707–1714. 18. , Jia, X. , and Finer, M. H. (1995) A packaging cell line for propagation of recombinant adenovirus vectors containing two lethal gene-region deletions.

217, 581–599. 15. , et al. (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263–267. 20 Fluri and Fussenegger 16. Bukovsky, A. , Song, J. , and Naldini, L. (1999) Interaction of human immunodeficiency virus-derived vectors with wild-type virus in transduced cells. J. Virol. 73, 7087–7089. 17. Danthinne, X. and Imperiale, M. J. (2000) Production of first generation adenovirus vectors: a review. Gene Ther. 7, 1707–1714. 18. , Jia, X. , and Finer, M.

And Kay, M. A. (2001) Extrachromosomal recombinant adeno-associated virus vector genomes are primarily responsible for stable liver transduction in vivo. J. Virol. 75, 6969–6976. 27. Samulski, R. , Chang, L. , and Shenk, T. (1989) Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression. J. Virol. 63, 3822–3828. 28. Hermonat, P. L. and Muzyczka, N. (1984) Use of adeno-associated virus as a mammalian DNA cloning vector: transduction of neomycin resistance into mammalian tissue culture cells.

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Animal Cell Biotechnology: Methods and Protocols (Methods in Biotechnology) by Ralf Pörtner


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